Abstract Scrub typhus is an important arthropod-borne disease causing significant acute febrile illness by infection with Orientia spp.Using a risk-based approach, this review Sleeping Aids examines current practice, the evidence base and regulatory requirements regarding matters of biosafety and biosecurity, and presents the case for reclassification from Risk Group 3 to Risk Group 2 along with recommendations for safe working practices of risk-based activities during the manipulation of Orientia spp.in the laboratory.
We recommend to reclassify Orientia spp.to Risk Group 2 based on the classification for RG2 pathogens as being moderate individual risk, low community risk.We recommend that low risk activities, can be performed within a biological safety cabinet located in a Biosafety Level (BSL) 2 core laboratory using standard personal protective equipment.
But when the risk assessment indicates, such as high Feeding concentration and volume, or aerosol generation, then a higher biocontainment level is warranted.For, the majority of animal activities involving Orientia spp., Animal BSL 2 (ABSL2) is recommended however where high risk activities are performed including necropsies, Animal BSL (ABSL3) is recommended.